You can also follow us on. If the ovary is chopped up into tiny pieces however, more water can be extracted. They are truly in suspended animation. To successfully freeze cells we must remove cell water (water expansion during freezing would burst the cell) and replace the water inside the cell with antifreeze.
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During freezing, sperm are cooled to a very low sub zero temperature (minus 196 degrees centigrade). Two frozen embryo transfer (fet) cycles were performed using hormone replacement protocol.